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[Validation of the perceived stress questionnaire(psq) in a german sample.]

After putting the finished reaction mixture in a heat cycler, the following procedure was run: primer annealing for 10 min at 25 C, reverse transcription for 15 min at 42 C, and inactivation for 5 min at 85 C

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Component scores are indicated as percentages

The biochemical characteristics of ferroptosis are mainly manifested as the accumulation of intracellular iron, the production of reactive oxygen species (ROS), the increase of lipid peroxides, and the decreased expression of glutathione peroxidase 4 in antioxidant system (glutathione system) [21]